Week 19 consisted of gathering data using the pond water as the growth media as opposed to the nutrient broth. This was done to see the difference between a so-called "perfect" scenario (the nutrient agar) and an imperfect "real" scenario (the pond water) it is a little difficult to see any results simply from the images as all the non-coliformal colonies grew up as well on the plate, however, we counted it against a control plate of un-infected pond water growth to count out any non-E.Coli B colonies.
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For week 18, the dilution set assay was re-run to prove its accuracy and correctness. The secondary results were just as promising as the first, with significant killing of E.Coli B seen in all dilutions, with a cleared, or almost cleared, solution in the 50, 5, and .5 cells/mL dilutions which are the most equivalent of an actual lake environment (shown in pictures 4-9). In the coming weeks after spring break, we will be running these assays with the actual pond water to see if the results are re-creatable in the real-life solution in which the interaction would be taking place. Also, distribution and delivery methods will be theorized and possibly tested before wrapping up experimentation.
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